Cat.No.702-rbCpH | 50 µg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50µl H2O to get a 1mg/ml solution of antibody in PBS.Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt!Avoid bright light to minimize photo bleeching of the fluorescent dye. |
Applications | ICC:yes (see remarks) gallery |
Label | CypHer5E |
Reactivity | Reacts with: rabbit.Other species not tested yet. |
Remarks | Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency. The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.ICC:Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark.Adjust the antibody to the desired dilution and immediately use it for your internalization assay. |
Data sheet | 702-rbcph.pdf |
1997年,马克斯-普朗克哥廷根研究所的著名科学家创立了Synaptic Systems。由于其针对突触蛋白的成熟,高质量抗体的需求量很大,我们开始向全球科学界提供这些研究试剂的业务。
在2002年,我们建立了自己的设备齐全的研究实验室,可以改进产品并开发新抗体。迄今为止,Synaptic Systems提供了针对突触和神经科学相关蛋白的最大抗体集合之一,以及用于细胞生物学,组织病理学和高分辨率显微镜的越来越多的抗体。这些抗体中的许多已在众多影响力大的出版物中确立了自己作为参考产品的地位。
多年来,我们的抗体产品组合已被精选的试剂盒和探针(如光不稳定的钙螯合剂)以及与下游免疫染色应用兼容的独特和创新的可固定膜染料(mCLINGs)所补充。
2017年,我们推出了 HistoSure 产品线。HistoSure抗体已专门开发用于福尔马林固定石蜡包埋的组织。像所有SYSY产品一样,HistoSure抗体仅可通过Synaptic Systems品牌获得。它们在我们自己的工厂中进行生产和全面测试,以确保最佳的质量和可靠性。HistoSure抗体受我们特殊的HistoSure质量指南的约束(请参阅: HistoSure抗体的验证)。
我们的Synaptic Systems抗体开发管线也可作为 定制单克隆抗体服务提供, 以探索可满足您的需求和预算的量身定制的抗体解决方案。
与学术机构和客户的紧密合作始终是将我们高技能员工的经验与合作伙伴在特定研究领域的专业知识相结合的首要任务。